Construct: shRNA TRCN0000243583

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001004164.2-3243s21c1
DNA Barcode:
AGATCCACAAAGCCTATAAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gnptab (432486)
Gene Description:
N-acetylglucosamine-1-phosphate transferase, alpha and beta subunits
Transcript:
RefSeq NM_001004164.2 (NON-CURRENT)
Match location:
Position 3243 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000243583)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGATCCACAAAGCCTATAAAG
Hairpin Sequence:
5'-CCGG-AGATCCACAAAGCCTATAAAG-CTCGAG-CTTTATAGGCTTTGTGGATCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGATCCACAAAGCCTATAAAGCTCGAGCTTTATAGGCTTTGTGGATCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGATCCACAAAGCCTATAAAGCTCGAGCTTTATAGGCTTTGTGGATCT-3'

Other clones with same target sequence:

(none)