Construct: shRNA TRCN0000243627

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_495855.4-712s21c1
DNA Barcode:
ATCTGGTTTCAGAATCGAAGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DUX4L12 (440014)
Gene Description:
double homeobox 4 like 12 (pseudogene)
Transcript:
RefSeq XM_495855.4 (NON-CURRENT)
Match location:
Position 712 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000243627)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCTGGTTTCAGAATCGAAGG
Hairpin Sequence:
5'-CCGG-ATCTGGTTTCAGAATCGAAGG-CTCGAG-CCTTCGATTCTGAAACCAGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCTGGTTTCAGAATCGAAGGCTCGAGCCTTCGATTCTGAAACCAGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCTGGTTTCAGAATCGAAGGCTCGAGCCTTCGATTCTGAAACCAGAT-3'

Other clones with same target sequence:

(none)