Construct: shRNA TRCN0000243675

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001099637.2-3828s21c1
DNA Barcode:
GGCGCTTTCCTACTGATTATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cep170 (545389)
Gene Description:
centrosomal protein 170
Transcript:
RefSeq NM_001099637.2 (CURRENT)
Match location:
Position 3828 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000243675)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCGCTTTCCTACTGATTATG
Hairpin Sequence:
5'-CCGG-GGCGCTTTCCTACTGATTATG-CTCGAG-CATAATCAGTAGGAAAGCGCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCGCTTTCCTACTGATTATGCTCGAGCATAATCAGTAGGAAAGCGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCGCTTTCCTACTGATTATGCTCGAGCATAATCAGTAGGAAAGCGCC-3'

Other clones with same target sequence:

  1. TRCN0000296286