Construct: shRNA TRCN0000243678

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001099637.2-3973s21c1
DNA Barcode:
TTCATTCAAGCACCGAATTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cep170 (545389)
Gene Description:
centrosomal protein 170
Transcript:
RefSeq NM_001099637.2 (CURRENT)
Match location:
Position 3973 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000243678)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCATTCAAGCACCGAATTAA
Hairpin Sequence:
5'-CCGG-TTCATTCAAGCACCGAATTAA-CTCGAG-TTAATTCGGTGCTTGAATGAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCATTCAAGCACCGAATTAACTCGAGTTAATTCGGTGCTTGAATGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCATTCAAGCACCGAATTAACTCGAGTTAATTCGGTGCTTGAATGAA-3'

Other clones with same target sequence:

(none)