Construct: shRNA TRCN0000243814

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001719017.1-860s21c1
DNA Barcode:
CTAATCCTATGGATGACAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC651789 (651789)
Gene Description:
similar to poly(A) binding protein interacting protein 1 isoform 1
Transcript:
RefSeq XM_001719017.1 (NON-CURRENT)
Match location:
Position 860 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000243814)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTAATCCTATGGATGACAATT
Hairpin Sequence:
5'-CCGG-CTAATCCTATGGATGACAATT-CTCGAG-AATTGTCATCCATAGGATTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTAATCCTATGGATGACAATTCTCGAGAATTGTCATCCATAGGATTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTAATCCTATGGATGACAATTCTCGAGAATTGTCATCCATAGGATTAG-3'

Other clones with same target sequence:

(none)