Construct: shRNA TRCN0000243826

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001123375.1-230s21c1
DNA Barcode:
AGGACTTTAAGACGGACCTGC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
H3C13 (653604)
Gene Description:
H3 clustered histone 13
Transcript:
RefSeq NM_001123375.1 (NON-CURRENT)
Match location:
Position 230 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000243826)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGACTTTAAGACGGACCTGC
Hairpin Sequence:
5'-CCGG-AGGACTTTAAGACGGACCTGC-CTCGAG-GCAGGTCCGTCTTAAAGTCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGACTTTAAGACGGACCTGCCTCGAGGCAGGTCCGTCTTAAAGTCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGACTTTAAGACGGACCTGCCTCGAGGCAGGTCCGTCTTAAAGTCCT-3'

Other clones with same target sequence:

(none)