Construct: shRNA TRCN0000243913

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182929.2-347s21c1
DNA Barcode:
GCTCCGAGGGCACGTTTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rims3 (242662)
Gene Description:
regulating synaptic membrane exocytosis 3
Transcript:
RefSeq NM_182929.2 (CURRENT)
Match location:
Position 347 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000243913)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCCGAGGGCACGTTTATTT
Hairpin Sequence:
5'-CCGG-GCTCCGAGGGCACGTTTATTT-CTCGAG-AAATAAACGTGCCCTCGGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCCGAGGGCACGTTTATTTCTCGAGAAATAAACGTGCCCTCGGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCCGAGGGCACGTTTATTTCTCGAGAAATAAACGTGCCCTCGGAGC-3'

Other clones with same target sequence:

(none)