Construct: shRNA TRCN0000243934

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152751.2-1837s21c1
DNA Barcode:
AGCAAGAGTGGACTGATTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BEND7 (222389)
Gene Description:
BEN domain containing 7
Transcript:
RefSeq NM_152751.2 (CURRENT)
Match location:
Position 1837 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000243934)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCAAGAGTGGACTGATTAAA
Hairpin Sequence:
5'-CCGG-AGCAAGAGTGGACTGATTAAA-CTCGAG-TTTAATCAGTCCACTCTTGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCAAGAGTGGACTGATTAAACTCGAGTTTAATCAGTCCACTCTTGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCAAGAGTGGACTGATTAAACTCGAGTTTAATCAGTCCACTCTTGCT-3'

Other clones with same target sequence:

(none)