Construct: shRNA TRCN0000243938

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017577.4-3524s21c1
DNA Barcode:
AGCAAGAATGAGTGGATTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GRAMD1C (54762)
Gene Description:
GRAM domain containing 1C
Transcript:
RefSeq NM_017577.4 (NON-CURRENT)
Match location:
Position 3524 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000243938)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCAAGAATGAGTGGATTATA
Hairpin Sequence:
5'-CCGG-AGCAAGAATGAGTGGATTATA-CTCGAG-TATAATCCACTCATTCTTGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCAAGAATGAGTGGATTATACTCGAGTATAATCCACTCATTCTTGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCAAGAATGAGTGGATTATACTCGAGTATAATCCACTCATTCTTGCT-3'

Other clones with same target sequence:

(none)