Construct: shRNA TRCN0000243947

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015268.3-5728s21c1
DNA Barcode:
TAGGTCCAAAGGTTCGAATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DNAJC13 (23317)
Gene Description:
DnaJ heat shock protein family (Hsp40) member C13
Transcript:
RefSeq NM_015268.3 (NON-CURRENT)
Match location:
Position 5728 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000243947)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAGGTCCAAAGGTTCGAATTA
Hairpin Sequence:
5'-CCGG-TAGGTCCAAAGGTTCGAATTA-CTCGAG-TAATTCGAACCTTTGGACCTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAGGTCCAAAGGTTCGAATTACTCGAGTAATTCGAACCTTTGGACCTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAGGTCCAAAGGTTCGAATTACTCGAGTAATTCGAACCTTTGGACCTA-3'

Other clones with same target sequence:

(none)