Construct: shRNA TRCN0000243971

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138360.3-2886s21c1
DNA Barcode:
AGAAACGCTGCCGCAAGATTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CARMIL3 (90668)
Gene Description:
capping protein regulator and myosin 1 linker 3
Transcript:
RefSeq NM_138360.3 (NON-CURRENT)
Match location:
Position 2886 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000243971)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAAACGCTGCCGCAAGATTC
Hairpin Sequence:
5'-CCGG-AGAAACGCTGCCGCAAGATTC-CTCGAG-GAATCTTGCGGCAGCGTTTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAAACGCTGCCGCAAGATTCCTCGAGGAATCTTGCGGCAGCGTTTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAAACGCTGCCGCAAGATTCCTCGAGGAATCTTGCGGCAGCGTTTCT-3'

Other clones with same target sequence:

(none)