Construct: shRNA TRCN0000243982

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178348.1-104s21c1
DNA Barcode:
CTAAGTGCCCTCCAGTCTCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LCE1A (353131)
Gene Description:
late cornified envelope 1A
Transcript:
RefSeq NM_178348.1 (NON-CURRENT)
Match location:
Position 104 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000243982)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTAAGTGCCCTCCAGTCTCTT
Hairpin Sequence:
5'-CCGG-CTAAGTGCCCTCCAGTCTCTT-CTCGAG-AAGAGACTGGAGGGCACTTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTAAGTGCCCTCCAGTCTCTTCTCGAGAAGAGACTGGAGGGCACTTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTAAGTGCCCTCCAGTCTCTTCTCGAGAAGAGACTGGAGGGCACTTAG-3'

Other clones with same target sequence:

(none)