Construct: shRNA TRCN0000244004

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144669.1-377s21c1
DNA Barcode:
GTCGCTTTCACAGAGTCAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GLT1D1 (144423)
Gene Description:
glycosyltransferase 1 domain containing 1
Transcript:
RefSeq NM_144669.1 (NON-CURRENT)
Match location:
Position 377 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000244004)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCGCTTTCACAGAGTCAATG
Hairpin Sequence:
5'-CCGG-GTCGCTTTCACAGAGTCAATG-CTCGAG-CATTGACTCTGTGAAAGCGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCGCTTTCACAGAGTCAATGCTCGAGCATTGACTCTGTGAAAGCGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCGCTTTCACAGAGTCAATGCTCGAGCATTGACTCTGTGAAAGCGAC-3'

Other clones with same target sequence:

(none)