Construct: shRNA TRCN0000244013

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181607.1-172s21c1
DNA Barcode:
GGCTTTGGAGGCTACGGATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KRTAP19-1 (337882)
Gene Description:
keratin associated protein 19-1
Transcript:
RefSeq NM_181607.1 (NON-CURRENT)
Match location:
Position 172 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000244013)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCTTTGGAGGCTACGGATAT
Hairpin Sequence:
5'-CCGG-GGCTTTGGAGGCTACGGATAT-CTCGAG-ATATCCGTAGCCTCCAAAGCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCTTTGGAGGCTACGGATATCTCGAGATATCCGTAGCCTCCAAAGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCTTTGGAGGCTACGGATATCTCGAGATATCCGTAGCCTCCAAAGCC-3'

Other clones with same target sequence:

(none)