Construct: shRNA TRCN0000244029

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003717.2-407s21c1
DNA Barcode:
GGGATCCTGGAGGAATGAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NPFF (8620)
Gene Description:
neuropeptide FF-amide peptide precursor
Transcript:
RefSeq NM_003717.2 (NON-CURRENT)
Match location:
Position 407 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000244029)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGGATCCTGGAGGAATGAATG
Hairpin Sequence:
5'-CCGG-GGGATCCTGGAGGAATGAATG-CTCGAG-CATTCATTCCTCCAGGATCCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGGATCCTGGAGGAATGAATGCTCGAGCATTCATTCCTCCAGGATCCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGGATCCTGGAGGAATGAATGCTCGAGCATTCATTCCTCCAGGATCCC-3'

Other clones with same target sequence:

(none)