Construct: shRNA TRCN0000244038

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053053.3-1100s21c1
DNA Barcode:
ACGAAACTCTGGCATCCAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TADA1 (117143)
Gene Description:
transcriptional adaptor 1
Transcript:
RefSeq NM_053053.3 (NON-CURRENT)
Match location:
Position 1100 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000244038)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGAAACTCTGGCATCCAAAT
Hairpin Sequence:
5'-CCGG-ACGAAACTCTGGCATCCAAAT-CTCGAG-ATTTGGATGCCAGAGTTTCGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGAAACTCTGGCATCCAAATCTCGAGATTTGGATGCCAGAGTTTCGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGAAACTCTGGCATCCAAATCTCGAGATTTGGATGCCAGAGTTTCGT-3'

Other clones with same target sequence:

(none)