Construct: shRNA TRCN0000244049

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138295.2-836s21c1
DNA Barcode:
TGGCCATCCTAGCTCGAAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PKD1L1 (168507)
Gene Description:
polycystin 1 like 1, transient receptor potential channel interacting
Transcript:
RefSeq NM_138295.2 (NON-CURRENT)
Match location:
Position 836 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000244049)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGCCATCCTAGCTCGAAATT
Hairpin Sequence:
5'-CCGG-TGGCCATCCTAGCTCGAAATT-CTCGAG-AATTTCGAGCTAGGATGGCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGCCATCCTAGCTCGAAATTCTCGAGAATTTCGAGCTAGGATGGCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGCCATCCTAGCTCGAAATTCTCGAGAATTTCGAGCTAGGATGGCCA-3'

Other clones with same target sequence:

(none)