Construct: shRNA TRCN0000244128

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031916.3-835s21c1
DNA Barcode:
ATCCTACCTTGCCTCTCTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ROPN1L (83853)
Gene Description:
rhophilin associated tail protein 1 like
Transcript:
RefSeq NM_031916.3 (NON-CURRENT)
Match location:
Position 835 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000244128)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCCTACCTTGCCTCTCTAAA
Hairpin Sequence:
5'-CCGG-ATCCTACCTTGCCTCTCTAAA-CTCGAG-TTTAGAGAGGCAAGGTAGGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCCTACCTTGCCTCTCTAAACTCGAGTTTAGAGAGGCAAGGTAGGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCCTACCTTGCCTCTCTAAACTCGAGTTTAGAGAGGCAAGGTAGGAT-3'

Other clones with same target sequence:

(none)