Construct: shRNA TRCN0000244164

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178123.4-537s21c1
DNA Barcode:
CAACAAATTGACTCGTTATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SESTD1 (91404)
Gene Description:
SEC14 and spectrin domain containing 1
Transcript:
RefSeq NM_178123.4 (NON-CURRENT)
Match location:
Position 537 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000244164)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACAAATTGACTCGTTATAT
Hairpin Sequence:
5'-CCGG-CAACAAATTGACTCGTTATAT-CTCGAG-ATATAACGAGTCAATTTGTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACAAATTGACTCGTTATATCTCGAGATATAACGAGTCAATTTGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACAAATTGACTCGTTATATCTCGAGATATAACGAGTCAATTTGTTG-3'

Other clones with same target sequence:

(none)