Construct: shRNA TRCN0000244189

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006346.2-1394s21c1
DNA Barcode:
AGCATCCAGAGACCATTATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIBF1 (10464)
Gene Description:
progesterone immunomodulatory binding factor 1
Transcript:
RefSeq NM_006346.2 (NON-CURRENT)
Match location:
Position 1394 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000244189)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCATCCAGAGACCATTATAA
Hairpin Sequence:
5'-CCGG-AGCATCCAGAGACCATTATAA-CTCGAG-TTATAATGGTCTCTGGATGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCATCCAGAGACCATTATAACTCGAGTTATAATGGTCTCTGGATGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCATCCAGAGACCATTATAACTCGAGTTATAATGGTCTCTGGATGCT-3'

Other clones with same target sequence:

(none)