Construct: shRNA TRCN0000244235

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013548.3-654s21c1
DNA Barcode:
AGATCGCGCAGGACTTCAAGA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hist1h3f (260423)
Gene Description:
histone cluster 1, H3f
Transcript:
RefSeq NM_013548.3 (NON-CURRENT)
Match location:
Position 654 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000244235)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGATCGCGCAGGACTTCAAGA
Hairpin Sequence:
5'-CCGG-AGATCGCGCAGGACTTCAAGA-CTCGAG-TCTTGAAGTCCTGCGCGATCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGATCGCGCAGGACTTCAAGACTCGAGTCTTGAAGTCCTGCGCGATCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGATCGCGCAGGACTTCAAGACTCGAGTCTTGAAGTCCTGCGCGATCT-3'

Other clones with same target sequence:

(none)