Construct: shRNA TRCN0000244258

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027444.2-972s21c1
DNA Barcode:
GGACTTGCAGGCCTGATATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bbx (70508)
Gene Description:
bobby sox HMG box containing
Transcript:
RefSeq NM_027444.2 (NON-CURRENT)
Match location:
Position 972 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000244258)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGACTTGCAGGCCTGATATTT
Hairpin Sequence:
5'-CCGG-GGACTTGCAGGCCTGATATTT-CTCGAG-AAATATCAGGCCTGCAAGTCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGACTTGCAGGCCTGATATTTCTCGAGAAATATCAGGCCTGCAAGTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGACTTGCAGGCCTGATATTTCTCGAGAAATATCAGGCCTGCAAGTCC-3'

Other clones with same target sequence:

(none)