Construct: shRNA TRCN0000244316

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007855.2-491s21c1
DNA Barcode:
TAGACTTCCTCTACCAGGTTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Twist2 (13345)
Gene Description:
twist basic helix-loop-helix transcription factor 2
Transcript:
RefSeq NM_007855.2 (NON-CURRENT)
Match location:
Position 491 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000244316)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAGACTTCCTCTACCAGGTTC
Hairpin Sequence:
5'-CCGG-TAGACTTCCTCTACCAGGTTC-CTCGAG-GAACCTGGTAGAGGAAGTCTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAGACTTCCTCTACCAGGTTCCTCGAGGAACCTGGTAGAGGAAGTCTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAGACTTCCTCTACCAGGTTCCTCGAGGAACCTGGTAGAGGAAGTCTA-3'

Other clones with same target sequence:

(none)