Construct: shRNA TRCN0000244342

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001002646.1-320s21c1
DNA Barcode:
CTGGAGAGAAACCCTACAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
EG668616 (668616)
Gene Description:
predicted gene, EG668616
Transcript:
RefSeq XM_001002646.1 (NON-CURRENT)
Match location:
Position 320 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000244342)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGGAGAGAAACCCTACAAAT
Hairpin Sequence:
5'-CCGG-CTGGAGAGAAACCCTACAAAT-CTCGAG-ATTTGTAGGGTTTCTCTCCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGGAGAGAAACCCTACAAATCTCGAGATTTGTAGGGTTTCTCTCCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGGAGAGAAACCCTACAAATCTCGAGATTTGTAGGGTTTCTCTCCAG-3'

Other clones with same target sequence:

  1. TRCN0000096537
  2. TRCN0000235358