Construct: shRNA TRCN0000244366

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030612.2-1764s21c1
DNA Barcode:
TCATGGGAAGCCCGATGAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nfkbiz (80859)
Gene Description:
nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, zeta
Transcript:
RefSeq NM_030612.2 (NON-CURRENT)
Match location:
Position 1764 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000244366)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCATGGGAAGCCCGATGAATA
Hairpin Sequence:
5'-CCGG-TCATGGGAAGCCCGATGAATA-CTCGAG-TATTCATCGGGCTTCCCATGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCATGGGAAGCCCGATGAATACTCGAGTATTCATCGGGCTTCCCATGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCATGGGAAGCCCGATGAATACTCGAGTATTCATCGGGCTTCCCATGA-3'

Other clones with same target sequence:

(none)