Construct: shRNA TRCN0000244417

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000203.3-519s21c1
DNA Barcode:
AGGACAAGCAGCAGGTGTTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IDUA (3425)
Gene Description:
alpha-L-iduronidase
Transcript:
RefSeq NM_000203.3 (NON-CURRENT)
Match location:
Position 519 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000244417)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGACAAGCAGCAGGTGTTTG
Hairpin Sequence:
5'-CCGG-AGGACAAGCAGCAGGTGTTTG-CTCGAG-CAAACACCTGCTGCTTGTCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGACAAGCAGCAGGTGTTTGCTCGAGCAAACACCTGCTGCTTGTCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGACAAGCAGCAGGTGTTTGCTCGAGCAAACACCTGCTGCTTGTCCT-3'

Other clones with same target sequence:

(none)