Construct: shRNA TRCN0000244434

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003185.3-2683s21c1
DNA Barcode:
CATCCAGATGTAGTAAGTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TAF4 (6874)
Gene Description:
TATA-box binding protein associated factor 4
Transcript:
RefSeq NM_003185.3 (CURRENT)
Match location:
Position 2683 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000244434)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCCAGATGTAGTAAGTTAT
Hairpin Sequence:
5'-CCGG-CATCCAGATGTAGTAAGTTAT-CTCGAG-ATAACTTACTACATCTGGATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCCAGATGTAGTAAGTTATCTCGAGATAACTTACTACATCTGGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCCAGATGTAGTAAGTTATCTCGAGATAACTTACTACATCTGGATG-3'

Other clones with same target sequence:

(none)