Construct: shRNA TRCN0000244436

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002912.3-6014s21c1
DNA Barcode:
TGACCTGTCTGAGACTATTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
REV3L (5980)
Gene Description:
REV3 like, DNA directed polymerase zeta catalytic subunit
Transcript:
RefSeq NM_002912.3 (NON-CURRENT)
Match location:
Position 6014 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000244436)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGACCTGTCTGAGACTATTTA
Hairpin Sequence:
5'-CCGG-TGACCTGTCTGAGACTATTTA-CTCGAG-TAAATAGTCTCAGACAGGTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGACCTGTCTGAGACTATTTACTCGAGTAAATAGTCTCAGACAGGTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGACCTGTCTGAGACTATTTACTCGAGTAAATAGTCTCAGACAGGTCA-3'

Other clones with same target sequence:

(none)