Construct: shRNA TRCN0000244441

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003297.2-888s21c1
DNA Barcode:
AGGACCTTCGTAGCCCATTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NR2C1 (7181)
Gene Description:
nuclear receptor subfamily 2 group C member 1
Transcript:
RefSeq NM_003297.2 (NON-CURRENT)
Match location:
Position 888 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000244441)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGACCTTCGTAGCCCATTAA
Hairpin Sequence:
5'-CCGG-AGGACCTTCGTAGCCCATTAA-CTCGAG-TTAATGGGCTACGAAGGTCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGACCTTCGTAGCCCATTAACTCGAGTTAATGGGCTACGAAGGTCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGACCTTCGTAGCCCATTAACTCGAGTTAATGGGCTACGAAGGTCCT-3'

Other clones with same target sequence:

(none)