Construct: shRNA TRCN0000244485

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023284.3-2131s21c1
DNA Barcode:
CATTCAACCTTCCCTACATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nuf2 (66977)
Gene Description:
NUF2, NDC80 kinetochore complex component
Transcript:
RefSeq NM_023284.3 (CURRENT)
Match location:
Position 2131 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000244485)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATTCAACCTTCCCTACATAT
Hairpin Sequence:
5'-CCGG-CATTCAACCTTCCCTACATAT-CTCGAG-ATATGTAGGGAAGGTTGAATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATTCAACCTTCCCTACATATCTCGAGATATGTAGGGAAGGTTGAATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATTCAACCTTCCCTACATATCTCGAGATATGTAGGGAAGGTTGAATG-3'

Other clones with same target sequence:

(none)