Construct: shRNA TRCN0000244539

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_358888.5-1018s21c1
DNA Barcode:
ATCGCTGAGGAGATAGGTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Paxbp1 (67367)
Gene Description:
PAX3 and PAX7 binding protein 1
Transcript:
RefSeq XM_358888.5 (NON-CURRENT)
Match location:
Position 1018 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000244539)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCGCTGAGGAGATAGGTATT
Hairpin Sequence:
5'-CCGG-ATCGCTGAGGAGATAGGTATT-CTCGAG-AATACCTATCTCCTCAGCGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCGCTGAGGAGATAGGTATTCTCGAGAATACCTATCTCCTCAGCGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCGCTGAGGAGATAGGTATTCTCGAGAATACCTATCTCCTCAGCGAT-3'

Other clones with same target sequence:

(none)