Construct: shRNA TRCN0000244590

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032036.2-164s21c1
DNA Barcode:
CTCCATAGCAGCCAAGATGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IFI27L2 (83982)
Gene Description:
interferon alpha inducible protein 27 like 2
Transcript:
RefSeq NM_032036.2 (NON-CURRENT)
Match location:
Position 164 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000244590)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCCATAGCAGCCAAGATGAT
Hairpin Sequence:
5'-CCGG-CTCCATAGCAGCCAAGATGAT-CTCGAG-ATCATCTTGGCTGCTATGGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCCATAGCAGCCAAGATGATCTCGAGATCATCTTGGCTGCTATGGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCCATAGCAGCCAAGATGATCTCGAGATCATCTTGGCTGCTATGGAG-3'

Other clones with same target sequence:

(none)