Construct: shRNA TRCN0000244602

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182854.1-1164s21c1
DNA Barcode:
AGTGCAGTGGCTCAATTATGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SNX20 (124460)
Gene Description:
sorting nexin 20
Transcript:
RefSeq NM_182854.1 (NON-CURRENT)
Match location:
Position 1164 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000244602)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTGCAGTGGCTCAATTATGT
Hairpin Sequence:
5'-CCGG-AGTGCAGTGGCTCAATTATGT-CTCGAG-ACATAATTGAGCCACTGCACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTGCAGTGGCTCAATTATGTCTCGAGACATAATTGAGCCACTGCACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTGCAGTGGCTCAATTATGTCTCGAGACATAATTGAGCCACTGCACT-3'

Other clones with same target sequence:

(none)