Construct: shRNA TRCN0000244739

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000778.3-2071s21c1
DNA Barcode:
CTTGCTCTCCCAGGATCAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CYP4A11 (1579)
Gene Description:
cytochrome P450 family 4 subfamily A member 11
Transcript:
RefSeq NM_000778.3 (NON-CURRENT)
Match location:
Position 2071 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000244739)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTGCTCTCCCAGGATCAATT
Hairpin Sequence:
5'-CCGG-CTTGCTCTCCCAGGATCAATT-CTCGAG-AATTGATCCTGGGAGAGCAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTGCTCTCCCAGGATCAATTCTCGAGAATTGATCCTGGGAGAGCAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTGCTCTCCCAGGATCAATTCTCGAGAATTGATCCTGGGAGAGCAAG-3'

Other clones with same target sequence:

(none)