Construct: shRNA TRCN0000244760

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017806.2-705s21c1
DNA Barcode:
TCCAGGGTCTGCAAGCCTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LIME1 (54923)
Gene Description:
Lck interacting transmembrane adaptor 1
Transcript:
RefSeq NM_017806.2 (NON-CURRENT)
Match location:
Position 705 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000244760)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCAGGGTCTGCAAGCCTAAA
Hairpin Sequence:
5'-CCGG-TCCAGGGTCTGCAAGCCTAAA-CTCGAG-TTTAGGCTTGCAGACCCTGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCAGGGTCTGCAAGCCTAAACTCGAGTTTAGGCTTGCAGACCCTGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCAGGGTCTGCAAGCCTAAACTCGAGTTTAGGCTTGCAGACCCTGGA-3'

Other clones with same target sequence:

(none)