Construct: shRNA TRCN0000244778

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144586.5-2393s21c1
DNA Barcode:
AGCATGAGAACACAGTAAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LYPD1 (116372)
Gene Description:
LY6/PLAUR domain containing 1
Transcript:
RefSeq NM_144586.5 (NON-CURRENT)
Match location:
Position 2393 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000244778)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCATGAGAACACAGTAAATG
Hairpin Sequence:
5'-CCGG-AGCATGAGAACACAGTAAATG-CTCGAG-CATTTACTGTGTTCTCATGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCATGAGAACACAGTAAATGCTCGAGCATTTACTGTGTTCTCATGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCATGAGAACACAGTAAATGCTCGAGCATTTACTGTGTTCTCATGCT-3'

Other clones with same target sequence:

(none)