Construct: shRNA TRCN0000244803

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030578.2-217s21c1
DNA Barcode:
CCAGCGGTTTCTCGGAAAGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
B9D2 (80776)
Gene Description:
B9 domain containing 2
Transcript:
RefSeq NM_030578.2 (NON-CURRENT)
Match location:
Position 217 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000244803)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCGGTTTCTCGGAAAGTA
Hairpin Sequence:
5'-CCGG-CCAGCGGTTTCTCGGAAAGTA-CTCGAG-TACTTTCCGAGAAACCGCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCGGTTTCTCGGAAAGTACTCGAGTACTTTCCGAGAAACCGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCGGTTTCTCGGAAAGTACTCGAGTACTTTCCGAGAAACCGCTGG-3'

Other clones with same target sequence:

(none)