Construct: shRNA TRCN0000244809

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003802.2-200s21c1
DNA Barcode:
CCTGCTTTGTAGCGGATAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MYH13 (8735)
Gene Description:
myosin heavy chain 13
Transcript:
RefSeq NM_003802.2 (NON-CURRENT)
Match location:
Position 200 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000244809)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGCTTTGTAGCGGATAATA
Hairpin Sequence:
5'-CCGG-CCTGCTTTGTAGCGGATAATA-CTCGAG-TATTATCCGCTACAAAGCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGCTTTGTAGCGGATAATACTCGAGTATTATCCGCTACAAAGCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGCTTTGTAGCGGATAATACTCGAGTATTATCCGCTACAAAGCAGG-3'

Other clones with same target sequence:

(none)