Construct: shRNA TRCN0000244819

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032682.4-751s21c1
DNA Barcode:
GCAGCAAGTTAGTGGATTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FOXP1 (27086)
Gene Description:
forkhead box P1
Transcript:
RefSeq NM_032682.4 (NON-CURRENT)
Match location:
Position 751 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000244819)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCAAGTTAGTGGATTAAA
Hairpin Sequence:
5'-CCGG-GCAGCAAGTTAGTGGATTAAA-CTCGAG-TTTAATCCACTAACTTGCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCAAGTTAGTGGATTAAACTCGAGTTTAATCCACTAACTTGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCAAGTTAGTGGATTAAACTCGAGTTTAATCCACTAACTTGCTGC-3'

Other clones with same target sequence:

  1. TRCN0000015664
  2. TRCN0000321629