Construct: shRNA TRCN0000244864

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008576.2-3900s21c1
DNA Barcode:
CCGTGTAGAGTTCCGGGATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Abcc1 (17250)
Gene Description:
ATP-binding cassette, sub-family C (CFTR/MRP), member 1
Transcript:
RefSeq NM_008576.2 (NON-CURRENT)
Match location:
Position 3900 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000244864)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTGTAGAGTTCCGGGATTA
Hairpin Sequence:
5'-CCGG-CCGTGTAGAGTTCCGGGATTA-CTCGAG-TAATCCCGGAACTCTACACGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTGTAGAGTTCCGGGATTACTCGAGTAATCCCGGAACTCTACACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTGTAGAGTTCCGGGATTACTCGAGTAATCCCGGAACTCTACACGG-3'

Other clones with same target sequence:

(none)