Construct: shRNA TRCN0000244928

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001236.3-622s21c1
DNA Barcode:
CATGGGAGAGTGGTGAATATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CBR3 (874)
Gene Description:
carbonyl reductase 3
Transcript:
RefSeq NM_001236.3 (NON-CURRENT)
Match location:
Position 622 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000244928)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATGGGAGAGTGGTGAATATC
Hairpin Sequence:
5'-CCGG-CATGGGAGAGTGGTGAATATC-CTCGAG-GATATTCACCACTCTCCCATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATGGGAGAGTGGTGAATATCCTCGAGGATATTCACCACTCTCCCATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATGGGAGAGTGGTGAATATCCTCGAGGATATTCACCACTCTCCCATG-3'

Other clones with same target sequence:

(none)