Construct: shRNA TRCN0000244931

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027769.2-642s21c1
DNA Barcode:
TTCACCGAACAGAGGTTATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cpne3 (70568)
Gene Description:
copine III
Transcript:
RefSeq NM_027769.2 (NON-CURRENT)
Match location:
Position 642 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000244931)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCACCGAACAGAGGTTATAA
Hairpin Sequence:
5'-CCGG-TTCACCGAACAGAGGTTATAA-CTCGAG-TTATAACCTCTGTTCGGTGAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCACCGAACAGAGGTTATAACTCGAGTTATAACCTCTGTTCGGTGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCACCGAACAGAGGTTATAACTCGAGTTATAACCTCTGTTCGGTGAA-3'

Other clones with same target sequence:

(none)