Construct: shRNA TRCN0000244959

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145115.2-362s21c1
DNA Barcode:
CTCAGCAGCAGTTGGGTATTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZSCAN25 (221785)
Gene Description:
zinc finger and SCAN domain containing 25
Transcript:
RefSeq NM_145115.2 (NON-CURRENT)
Match location:
Position 362 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000244959)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCAGCAGCAGTTGGGTATTC
Hairpin Sequence:
5'-CCGG-CTCAGCAGCAGTTGGGTATTC-CTCGAG-GAATACCCAACTGCTGCTGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCAGCAGCAGTTGGGTATTCCTCGAGGAATACCCAACTGCTGCTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCAGCAGCAGTTGGGTATTCCTCGAGGAATACCCAACTGCTGCTGAG-3'

Other clones with same target sequence:

(none)