Construct: shRNA TRCN0000244965

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007222.3-950s21c1
DNA Barcode:
TCGGGAATATCTATCAGTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZHX1 (11244)
Gene Description:
zinc fingers and homeoboxes 1
Transcript:
RefSeq NM_007222.3 (NON-CURRENT)
Match location:
Position 950 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000244965)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCGGGAATATCTATCAGTAAA
Hairpin Sequence:
5'-CCGG-TCGGGAATATCTATCAGTAAA-CTCGAG-TTTACTGATAGATATTCCCGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCGGGAATATCTATCAGTAAACTCGAGTTTACTGATAGATATTCCCGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCGGGAATATCTATCAGTAAACTCGAGTTTACTGATAGATATTCCCGA-3'

Other clones with same target sequence:

(none)