Construct: shRNA TRCN0000244986

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007294.2-3465s21c1
DNA Barcode:
TTGCAACCTGAGGTCTATAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BRCA1 (672)
Gene Description:
BRCA1 DNA repair associated
Transcript:
RefSeq NM_007294.2 (NON-CURRENT)
Match location:
Position 3465 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000244986)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGCAACCTGAGGTCTATAAA
Hairpin Sequence:
5'-CCGG-TTGCAACCTGAGGTCTATAAA-CTCGAG-TTTATAGACCTCAGGTTGCAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGCAACCTGAGGTCTATAAACTCGAGTTTATAGACCTCAGGTTGCAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGCAACCTGAGGTCTATAAACTCGAGTTTATAGACCTCAGGTTGCAA-3'

Other clones with same target sequence:

(none)