Construct: shRNA TRCN0000244999

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004957.4-415s21c1
DNA Barcode:
GCCTGAAGACGGGATTCTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FPGS (2356)
Gene Description:
folylpolyglutamate synthase
Transcript:
RefSeq NM_004957.4 (NON-CURRENT)
Match location:
Position 415 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000244999)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGAAGACGGGATTCTTTA
Hairpin Sequence:
5'-CCGG-GCCTGAAGACGGGATTCTTTA-CTCGAG-TAAAGAATCCCGTCTTCAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGAAGACGGGATTCTTTACTCGAGTAAAGAATCCCGTCTTCAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGAAGACGGGATTCTTTACTCGAGTAAAGAATCCCGTCTTCAGGC-3'

Other clones with same target sequence:

(none)