Construct: shRNA TRCN0000245007

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000176.2-6190s21c1
DNA Barcode:
TTGGGTGGAGTTTCGTAATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NR3C1 (2908)
Gene Description:
nuclear receptor subfamily 3 group C member 1
Transcript:
RefSeq NM_000176.2 (NON-CURRENT)
Match location:
Position 6190 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000245007)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGGGTGGAGTTTCGTAATTT
Hairpin Sequence:
5'-CCGG-TTGGGTGGAGTTTCGTAATTT-CTCGAG-AAATTACGAAACTCCACCCAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGGGTGGAGTTTCGTAATTTCTCGAGAAATTACGAAACTCCACCCAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGGGTGGAGTTTCGTAATTTCTCGAGAAATTACGAAACTCCACCCAA-3'

Other clones with same target sequence:

(none)