Construct: shRNA TRCN0000245045

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030636.2-3556s21c1
DNA Barcode:
GGTCACACCACTGGGTAAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EEPD1 (80820)
Gene Description:
endonuclease/exonuclease/phosphatase family domain containing 1
Transcript:
RefSeq NM_030636.2 (NON-CURRENT)
Match location:
Position 3556 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000245045)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGTCACACCACTGGGTAAATA
Hairpin Sequence:
5'-CCGG-GGTCACACCACTGGGTAAATA-CTCGAG-TATTTACCCAGTGGTGTGACC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGTCACACCACTGGGTAAATACTCGAGTATTTACCCAGTGGTGTGACCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGTCACACCACTGGGTAAATACTCGAGTATTTACCCAGTGGTGTGACC-3'

Other clones with same target sequence:

(none)