Construct: shRNA TRCN0000245123

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003099.3-768s21c1
DNA Barcode:
GTACCTTCAGAGGATTGTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SNX1 (6642)
Gene Description:
sorting nexin 1
Transcript:
RefSeq NM_003099.3 (NON-CURRENT)
Match location:
Position 768 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000245123)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTACCTTCAGAGGATTGTAAA
Hairpin Sequence:
5'-CCGG-GTACCTTCAGAGGATTGTAAA-CTCGAG-TTTACAATCCTCTGAAGGTAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTACCTTCAGAGGATTGTAAACTCGAGTTTACAATCCTCTGAAGGTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTACCTTCAGAGGATTGTAAACTCGAGTTTACAATCCTCTGAAGGTAC-3'

Other clones with same target sequence:

(none)