Construct: shRNA TRCN0000245127

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033218.1-1218s21c1
DNA Barcode:
GCCATTGATTACATCAAATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Srebf2 (20788)
Gene Description:
sterol regulatory element binding factor 2
Transcript:
RefSeq NM_033218.1 (CURRENT)
Match location:
Position 1218 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000245127)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATTGATTACATCAAATAT
Hairpin Sequence:
5'-CCGG-GCCATTGATTACATCAAATAT-CTCGAG-ATATTTGATGTAATCAATGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATTGATTACATCAAATATCTCGAGATATTTGATGTAATCAATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATTGATTACATCAAATATCTCGAGATATTTGATGTAATCAATGGC-3'

Other clones with same target sequence:

  1. TRCN0000215753